Changes in deoxyribonucleic acid polymerase activities in synthesis of deoxyribonucleic acid during sporulation of Bacillus subtilis

A deoxyribonucleic acid polymerase I-deficient mutant of Bacillus subtilis.

A methyl methanesulfonate sensitive mutant of Bacillus subtilis appears to lack the enzyme DNA polymerase I. The absence of polymerase I permits the detection of a second DNA polymerizing enzyme in the mutant. This second polymerase is inhibitable by sulfhydryl antagonists, and shows maximal activity on double-stranded DNA substrates with many single strand breaks. Poly[d(A-T)] is less efficien...

Tests for deoxyribonucleic acid synthesis in toluenized Bacillus subtilis cells.

Several tests were devised to further characterize deoxyribonucleic acid (DNA) synthesis in toluenized Bacillus subtilis cells. Vigorous agitation of toluenized cells (localization test) demonstrated that the DNA replication is exclusively a cell-associated process. A DNA "repair" condition was also applied to toluenized cells and shown to be distinct from DNA replication in its DNA polymerase ...

Plasmid deoxyribonucleic acid in Bacillus subtilis and Bacillus pumilus.

Two of eighteen strains of Bacillus subtilis examined contained covalently closed circular duplex deoxyribonucleic acid (DNA) of homogeneous size and buoyant density. Strain ATCC 15841 contained about 16 copies per chromosome of plasmid pPL1, a circular DNA element having a molecular weight of about 4.7 times 10(6) and a buoyant density of 1.700. Strain ATCC 7003 contained about one to two copi...

Enzymatic Synthesis of Deoxyribonucleic Acid. Xv. Purification and Properties of a Polymerase from Bacillus Subtilis.

Variation of deoxyribonucleic acid polymerase activities during rat liver regeneration.

The existence of multiple molecular weight forms of DNA polymerase in different cellular locations of eukaryotie cells, and the demonstration that these enzyme activities have quite different properties (l-4) raise a number of questions about DNA metabolism in complex organisms. Some of these questions were implicit in the early work on mammalian DNA polymerase; particularly the demonstration t...